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R. Poorniammal ¹, S. P. Sundaram ¹, K. Kumutha ¹, R. Parimala Devi ¹

Affiliations
1 Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore (T.N), IN

Abstract

The effect of cocultured inoculant (Azophosmet) was studied under in vitro and in vivo conditions compared with other individual inoculants was investigated. The cotton seeds were treated with the Azophosmet, revealed the surviving ability of Azospirillum Sp7, PSB PB1 and PPFM CO 47 noticed up to 24 h on the seeds. Inoculation of cocultured inoculants recorded the maximum increase in ischolar_main length; shoot length, germination per cent and vigour index of cotton plants followed by CO 47 under in vitro conditions. Cocultured inoculants (Azophosmet) was effective in enhancing rhizosphere population of individual bioinoculants, plant growth and seed cotton yield under pot culture conditions.

Keywords

Azophosmet, Cotton, Coculturing and PPFM.

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R. Poorniammal ¹, S. P. Sundaram ¹, K. Kumutha ¹, R. Parimala Devi ¹

Affiliations
1 Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore (T.N.), IN

Abstract

Nitrogen fixers, phosphate solubilizers and a plant growth hormone synthesizer were cocultured in a suitable single medium The compatibility nature of Azospirillum brasilense Sp7, Bacillus megaterium var phosphaticum PB1 and Methylobacterium extorquens CO 47 in a common growth medium was investigated to coculture the bioinoculants (Azophosmet). All the three had better growth in YEMA. During coculturing the survival of Azospirillum Sp7, PSB PB1 and PPFM CO 47 was maximum in YEMB supplemented with 0.5% methanol. Biochemical characters of YEMB culture filtrates (0.5% methanol) recorded the highest amount of ammonia secretion, cell protein and polysaccharide content when compared to nutrient broth and glycerol peptone broth. The shelf life studies of Azophosmet in lignite carrier based formulation assessed for four months indicated that Azospirillum Sp7, PSB PB1 and PPFM CO 47 had cell load of 10⁸cfu g^-1. The cotton seeds treated with the Azophosmet revealed the surviving ability of Azospirillum Sp7, PSB PB1 and PPFM CO 47 noticed up to 24 hr on the seeds.

Keywords

Azospirillum, Bacillus, Methylobacterium and Coculturing.

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R. Parimala Devi ¹, S. P. Sundaram ¹, R. Poorniammal ¹

Affiliations
1 Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore (T.N.), IN

Abstract

The effect of facultative methylotrophs (FMs) on tissue culturing of rice cultivar Co43 was studied under in vitro conditions. Facultative methylotrophic isolates obtained from calli tissues of various tropical plants were screened based on growth hormone production and their effect on seedling vigour of rice cultivar Co43. Efficient isolates viz., PPFMs-LE1, PPFMs-OS, PPFMs-Co47, PPFMs-Vu, PPFMs-OS and NPFMs-OS along with standard strain Methylobacterium extorquens AM1 were inoculated by following various methods. Upon inoculation, the calli tissues were found to be well colonized by facultative methylotrophs and this led to proliferation of calli tissues and increased the regenerating ability of calli tissues. Chlorophyll and soluble protein content were also found to be increased significantly over the uninoculated control.

Keywords

Facultative Methylotrophs, Chlorophyll, Auxin, Cytokinins, Tissue Culture.

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